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KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody AGI2345
    Western blotting analysis using anti-Cyclin B1 antibody (Cat#69151). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin B1 antibody (Cat#69151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody AGI2345
    Western blotting analysis using anti-Cyclin B1 antibody (Cat#69151). Cyclin B1 expression in wild type (WT) and cyclin B1 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cyclin B1 antibody (Cat#69151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody AGI2345
    Flow cytometric analysis of Cyclin B1 expression in HepG2 cells using Cyclin B1 antibody (Cat#69151, 1:2,000). Green, isotype control; red, Cyclin B1.
    detail
  • ICC - KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody AGI2345
    Immunocytochemical staining of HepG2 cells with Cyclin B1 antibody (Cat#69151, 1:1,000). Nuclei were stained blue with DAPI; Cyclin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P14635
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 1670
Calculated MW Predicted, 48 kDa ; Observed, 55 kDa
Gene Name CCNB1
Aliases CCNB1; Cyclin B1; CCNB; G2/Mitotic-Specific Cyclin-B1; G2/Mitotic-Specific Cyclin B1
Immunogen A synthesized peptide derived from human Cyclin B1
Additional Information
Gene ID 891
Other Names G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Protein Information
Name CCNB1
Synonyms CCNB
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2345
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